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Biology · May / June 2026 · Paper 61 · Question 2

0610

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The question as text

Plan an investigation to determine the effect of temperature on the rate of respiration in yeast. [6]

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1 (independent variable)

at least two different temperatures ;

2 (dependent variable)

number / amount, of bubbles OR volume of, gas / carbon dioxide, produced ;

3 and 4 (method) ;;

gas syringe (for volume of gas) / collecting gas over

water / displacement

use of a thermostatically controlled water-bath
idea of time for equilibration before taking measurements
yeast added to, water / sugar solution OR yeast suspension
AVP (that is appropriate for the dependent variable given) 5, 6 & 7 (variables kept constant) ;;;
type / species / AW, of yeast
volume / mass / concentration, of yeast
volume / mass / concentration, of (named) sugar (solution)
measuring over set time period OR measuring time taken for set volume of gas to be produced (or other dependent variable)
pH

8 at least three trials / two more repeats ;

9 relevant safety precaution ;

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