Biology · Oct / Nov 2025 · Paper 61 · Question 2
0610
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The question as text
Amylase is an enzyme that catalyses the breakdown of starch to form reducing sugars. Plan an investigation to determine the effect of pH on the activity of amylase. Use iodine solution in your method. [6]
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total of six from:
1
independent variable
at least two pH values , tested / stated ;
2
dependent variable
time for iodine solution to stay yellow-brown / time for disappearance of starch/colour of iodine (solution) after a set period of time ;
3, 4 + 5 variables kept constant ;;;
6 & 7 method ;;
8
repeat investigation twice more / at least two replicates ;
9
relevant safety precaution ;
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