Biology · May / June 2021 · Paper 61 · Question 1
0610
As printed in the paper
The question as text
A student investigated the effect of the concentration of sugar solutions on osmosis in potato cells. Step 1 The student labelled four test-tubes A, B, C and D. Step 2 The volumes of 1 mol per dm3 sugar solution and distilled water shown in Table 1.1 were used to make solutions containing different concentrations of sugar in each test-tube.
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1(a)(i)[1]
0.4 ;
1(a)(ii)[3]
table drawn with a minimum of two, columns / row, and a header line ; headers with units ;
four correct lengths ±1 mm recorded ;
1(a)(iii)[1]
so that the results were comparable / so sugar concentration is the only variable that is changed / to ensure the surface area was the same / so surface area does not affect the rate of osmosis / AW ;
1(a)(iv)[1]
concentration of the sugar solution ;
1(a)(v)[2]
any two from:
measure mass of cylinders (instead of length) ;
more intermediate concentrations of sugar solution ;
extend the range of concentrations used ;
repeat at each concentration / replicate the investigation ;
soak for longer than 20 minutes ;
use warmer sugar solutions (to speed up diffusion) ;
keep all at the same temperature ;
dry cylinders before measuring ;
1(a)(vi)[1]
cut away from body / use wooden board or tile / cut on flat
surface / keep blade away from body parts / dry before cutting ;
1(b)[6]
independent variable:
1
ref. to using at least 2 different temperatures ;
method:
2
method of maintaining a temperature described ;
3
use of a tissue e.g. potato / leaves / root / stem, immersed in water / sugar / salt solution ;
4
method measuring dependent variable described, i.e.
length / mass / volume / surface area of (cylinders / tissue / leaves) before and after (immersion) or measuring volume of immersion liquid before and after ;
5 + 6 variables kept constant ;;
max two from:
same concentration (of solution) / same volume (of solution / water)same (initial) length / volume / mass / surface area, of potato or tissuesame type / age /variety / AW, of (potato, tissue) / same potato / same plantsame pH / use a buffersame immersion time7
equilibration of temperature for tissue / solution ;
8
drying before reweighing (if mass measured) ;
9
at least two repeats (of each temperature) / total of three trials ; 10 AVP ;
1(c)(i)[2]
iodine solution ;
blue-black ;
1(c)(ii)[5]
lines: clear, single, unbroken with no shading ;
size: central cell has a minimum diameter of 56 mm ;
detail one: double line (minimum) for entire cell wall ;
detail two: minimum of five starch grains around perimeter of cell ; label: one starch grain labelled ;
1(c)(iii)[3]
(length of line AB) 10 ±1 (mm) ;
0.013 (0.011–0.014) mm ;;
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