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Biology · May / June 2021 · Paper 41 · Question 3

0610

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The question as text

Bacteria are used in many biotechnological processes.

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  • 3(a)[3]

    any three from:

    (small so do) not need much space ;

    rapid reproduction rate / AW ;

    can make complex molecule(s) ;

    no ethical concerns over use ;

    idea that genetic code is universal ;

    they have plasmids ;

    idea that plasmids are used, as vectors / in genetic engineering ; simple requirements / AW, so, easy / cheap, to grow ;

    AVP ;

  • 3(b)(i)[1]

    pancreas ;

  • 3(b)(ii)[1]

    (type 1) diabetes ;

  • 3(b)(iii)[1]

    reduces blood sugar concentration ;

  • 3(c)(i)[1]

    1 y-axis = number / population, of (living) bacteria per (1.0) mm3 and

    2 x-axis = time / hours / days ;

  • 3(c)(ii)[2]

    P – lag

    Q – exponential / log

    R – stationary

    S – death / decline

    ;;

  • 3(c)(iii)[3]

    any three from:

    limiting factor(s) / factors that limit ;

    not enough space / overpopulation ;

    competition ;

    fewer / no, (named) nutrient(s) / food ;

    not enough oxygen ;

    change in pH / increase in acidity ;

    increase in temperature ;

    build-up of, (named) toxic / waste, substances ;

  • 3(d)[3]

    total of three from:

    importance of iron to max 2

    to make / for, haemoglobin ;

    to make / for, red blood cells ;

    for transport of oxygen ;

    AVP ;

    effects of iron deficiency

    (iron-deficiency) anaemia ;

    any symptom of anaemia ;

  • 3(e)(i)[1]

    restriction (enzyme) ;

  • 3(e)(ii)[2]

    any two from:

    use same (restriction) enzyme to cut cassava DNA ;

    sticky ends are, formed / joined ;

    ref. to complementary, ends / base pairs ;

    (joined by) ligase ;

  • 3(e)(iii)[2]

    any two from:

    idea that need to check that offspring inherit ‘new’ gene / trait ; ensure new (GM) variety can grow in field conditions / AW ;

    make sure the ‘new’ gene does not cause any adverse effects ; make sure (GM) plants have, the same / a high enough, yield ; idea of building up a large quantity of, seed / plants, to sell to farmers ; AVP ;

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